You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Sfp-Type 4'-Phosphopantetheinyl Transferase Is Indispensable for Fungal Pathogenicity
Ralf Horbach, Alexander Graf, Fabian Weihmann, Luis Antelo, Sebastian Mathea, Johannes C. Liermann, Till Opatz, Eckhard Thines, Jesús Aguirre and Holger B. Deising
The Plant Cell
Vol. 21, No. 10 (Oct., 2009), pp. 3379-3396
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/40537513
Page Count: 18
You can always find the topics here!Topics: Infections, Appressoria, Fungi, Corn, Plants, Leaves, Plant cells, Hyphae, Siderophores, Conidia
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
In filamentous fungi, Sfp-type 4'-phosphopantetheinyl transferases (PPTases) activate enzymes involved in primary (α-aminoadipate reductase [AAR]) and secondary (polyketide synthases and nonribosomal peptide synthetases) metabolism. We cloned the PPTase gene PPT1 of the maize anthracnose fungus Colletotrich urn graminicola and generated PPTasedeficient mutants [Δppt1). Δppti strains were auxotrophic for Lys, unable to synthesize siderophores, hypersensitive to reactive oxygen species, and unable to synthesize polyketides (PKs). A differential analysis of secondary metabolites produced by wild-type and Δppt1 strains led to the identification of six novel PKs. Infection-related morphogenesis was affected in Δppt1 strains. Rarely formed appressoria of Δppt1 strains were nonmelanized and ruptured on intact plant. The hyphae of Δppt1 strains colonized wounded maize (Zea mays) leaves but failed to generate necrotic anthracnose disease symptoms and were defective in asexual sporulation. To analyze the pleiotropic pathogenicity phenotype, we generated AAR-deficient mutants (Δaar1) and employed a melanin-deficient mutant (M1.502). Results indicated that PPT1 activates enzymes required at defined stages of infection. Melanization is required for cell wall rigidity and appressorium function, and Lys supplied by the AAR1 pathway is essential for necrotrophic development. As PPTase-deficient mutants of Magnaporthe oryzea were also nonpathogenic, we conclude that PPTases represent a novel fungal pathogenicity factor.
The Plant Cell © 2009 American Society of Plant Biologists (ASPB)