Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Myosin Light Chain Kinase (MLCK) Gene Disruption in Dictyostelium: A Role for MLCK-A in Cytokinesis and Evidence for Multiple MLCKs

Janet L. Smith, Linda A. Silveira and James A. Spudich
Proceedings of the National Academy of Sciences of the United States of America
Vol. 93, No. 22 (Oct. 29, 1996), pp. 12321-12326
Stable URL: http://www.jstor.org/stable/40615
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Myosin Light Chain Kinase (MLCK) Gene Disruption in Dictyostelium: A Role for MLCK-A in Cytokinesis and Evidence for Multiple MLCKs
Preview not available

Abstract

We have created a strain of Dictyostelium that is deficient for the Ca$^{2+}$/calmodulin-independent MLCK-A. This strain undergoes cytokinesis less efficiently than wild type, which results in an increased frequency of multinucleate cells when grown in suspension. The MLCK-A$^{-}$ cells are able, however, to undergo development and to cap crosslinked surface receptors, processes that require myosin heavy chain. Phosphorylated regulatory light chain (RLC) is still present in MLCK-A$^{-}$ cells, indicating that Dictyostelium has one or more additional protein kinases capable of phosphorylating RLC. Concanavalin A treatment was found to induce phosphorylation of essentially all of the RLC in wild-type cells, but RLC phosphorylation levels in MLCK-A$^{-}$ cells are unaffected by concanavalin A. Thus MLCK-A is regulated separately from the other MLCK(s) in the cell.

Page Thumbnails

  • Thumbnail: Page 
12321
    12321
  • Thumbnail: Page 
12322
    12322
  • Thumbnail: Page 
12323
    12323
  • Thumbnail: Page 
12324
    12324
  • Thumbnail: Page 
12325
    12325
  • Thumbnail: Page 
12326
    12326