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Influence of Nuclear Geometry on the Formation of Genetic Rearrangements in Human Cells

M. Durante, D. Pignalosa, J. A. Jansen, X. F. Walboomers and S. Ritter
Radiation Research
Vol. 174, No. 1 (July 2010), pp. 20-26
Stable URL: http://www.jstor.org/stable/40785256
Page Count: 7
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Influence of Nuclear Geometry on the Formation of Genetic Rearrangements in Human Cells
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Abstract

Interphase chromosomes are divided into discrete domains, with limited overlapping and movement. We explored the role of nuclear topology in the formation of chromosome aberrations by irradiating normal human fibroblasts with high-energy heavy ions from different directions. Cells with elliptical nuclei were grown in an aligned manner onto micrometer grooved culturing substrates to have a predetermined orientation with respect to the accelerated iron ions. Particles were directed either perpendicular to the cell layer or along the major or minor axis of the nucleus. Analysis of chromosome aberrations by mFISH showed that, at the same radiation dose, the yield of chromosomal damage and its complexity are largely modified by the irradiation geometry. The results demonstrate that the architecture of the cell nucleus determines the formation of rearrangements.

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