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Establishment of Culture Conditions for Survival of Histomonas meleagridis in Transit

R. W. Gerhold, L. A. Lollis, R. B. Beckstead and L. R. McDougald
Avian Diseases
Vol. 54, No. 2 (June 2010), pp. 948-950
Stable URL: http://www.jstor.org/stable/40801726
Page Count: 3
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Establishment of Culture Conditions for Survival of Histomonas meleagridis in Transit
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Abstract

Fresh ceca samples from turkeys in North Carolina infected with Histomonas meleagridis were collected at necropsy, inoculated into warmed Dwyers medium, and sent by overnight courier to our laboratory at The University of Georgia. Further incubation at 40 yielded positive cultures from all four samples. PCR and DNA sequencing confirmed the presence of H.meleagridis. To further establish conditions for survival in transit, we infected turkeys with H. meleagridis, euthanatized the birds 10 days postinfection, and allowed carcasses to incubate at room temperature for either 2 or 24 hr. After incubation, samples of cecal contents (0.5 g) were placed in Dwyers medium and held at 4, 25, or 30 for 6, 18, 24, 48, 72, 96, or 120 hr, simulating holding conditions during transit. Samples were placed in a 40 incubator at the specified times and examined daily for histomonad growth by light microscopy. Positive histomonad growth was detected from cecal samples obtained from the 2-hr incubated carcass and from cultures held at 30 for 6, 18, 24, 48, and 72 hr. No growth was seen from cultures held at 25 or 4 isolation can be made from field samples, provided that material is collected at warm temperatures and transported rapidly to the laboratory. Se recolectaron a la necropsia muestras frescas de contenido cecal de pavos de Carolina del Norte, que estaban infectados con Histomonas meleagridis, las muestras fueron inoculadas en medio Dwyers previamente calentado y se enviaron por correo urgente a nuestro laboratorio en la Universidad de Georgia. La incubación adicional a 40° resultó en cultivos positivos en las cuatro muestras. Mediante la reacción en cadena de la polimerasa y por secuenciación del ADN se confirmó la presencia de H. meleagridis. A fin de establecer las condiciones para la supervivencia durante el transporte, se infectaron pavos con H meleagridis, se practicó la eutanasia a las aves a los 10 días postinfección y se incubaron las canales a temperatura ambiente durante 2 ó 24 horas. Después de la incubación, las muestras de contenido cecal (0.5 g) se colocaron en medio Dwyers y se mantuvieron a 4 C, 25 C ó 30 C por 6, 18, 24, 48, 72, 96, ó 120 horas, simulando las condiciones durante el tránsito. Las muestras se colocaron en una incubadora a 40 crecimiento positivo de histomonas en muestras cecales obtenidas de las canales incubadas por dos horas y de los cultivos mantenidos a 30 por 6, 18, 24, 48 y 72 horas. No se observó crecimiento en los cultivos mantenidos a 25 C ó a 4 C o e n cualquier temperatura de las canales que se incubaron durante 24 horas a temperatura ambiente. Estos resultados sugieren que se puede obtener aislamientos positivos a partir de las muestras de campo, siempre que el material se recolecte a temperatura cálida y se transporte rápidamente al laboratorio.

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