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X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides
Michael L. Gleghorn, Elena K. Davydova, Ritwika Basu, Lucia B. Rothman-Denes, Katsuhiko S. Murakami and E. Peter Geiduschek
Proceedings of the National Academy of Sciences of the United States of America
Vol. 108, No. 9 (March 1, 2011), pp. 3566-3571
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/41060972
Page Count: 6
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We have determined the X-ray crystal structures of the pre-and postcatalytic forms of the initiation complex of bacteriophage N4 RNA polymerase that provide the complete set of atomic images depicting the process of transcript initiation by a single-subunit RNA polymerase. As observed during T7 RNA polymerase transcript elongation, substrate loading for the initiation process also drives a conformational change of the helix, but only the correct base pairing between the +2 substrate and DNA base is able to complete the O-helix conformational transition. Substrate binding also facilitates catalytic metal binding that leads to alignment of the reactive groups of substrates for the nucleotidyl transfer reaction. Although all nucleic acid polymerases use two divalent metals for catalysis, they differ in the requirements and the timing of binding of each metal. In the case of bacteriophage RNA polymerase, we propose that catalytic metal binding is the last step before the nucleotidyl transfer reaction.
Proceedings of the National Academy of Sciences of the United States of America © 2011 National Academy of Sciences