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Prospective separation of normal and leukemie stem cells based on differential expression of TIM3, a human acute myeloid leukemia stem cell marker
Max Jan, Mark P. Chao, Adriel C. Cha, Ash A. Alizadeh, Andrew J. Gentles, Irving L. Weissman, Ravindra Majeti and Peter K. Vogt
Proceedings of the National Academy of Sciences of the United States of America
Vol. 108, No. 12 (March 22, 2011), pp. 5009-5014
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/41125260
Page Count: 6
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Hematopoietic tissues in acute myeloid leukemia (AML) patients contain both leukemia stem cells (LSC) and residual normal hematopoietic stem cells (HSC). The ability to prospectively separate residual HSC from LSC would enable important scientific and clinical investigation including the possibility of purged autologous hematopoietic cell transplants. We report here the identification of TIM3 as an AML stem cell surface marker more highly expressed on multiple specimens of AML LSC than on normal bone marrow HSC. TIM3 expression was detected in all cytogenetic subgroups of AML, but was significantly higher in AML-associated with core binding factor translocations or mutations in By assessing engraftment in NOD/SCID/IL2Rγ-null mice, we determined that HSC function resides predominantly in the TIM3-negative fraction of normal bone marrow, whereas LSC function from multiple AML specimens resides predominantly in the TIM3-positive compartment. Significantly, differential TIM3 expression enabled the prospective separation of HSC from LSC in the majority of AML specimens with detectable residual HSC function.
Proceedings of the National Academy of Sciences of the United States of America © 2011 National Academy of Sciences