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Sequence Analyses of the Representative Chinese-Prevalent Strain of Avian Nephritis Virus in Healthy Chicken Flocks

W. Zhao, X. G. Hua, C. L. Yuan, L. Cui, T. L. Shan, X. Q. Dai, A. L. Zhu, Y. Yu, C. X. Zhu and Z. B. Yang
Avian Diseases
Vol. 55, No. 1 (March 2011), pp. 65-69
Stable URL: http://www.jstor.org/stable/41319297
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Sequence Analyses of the Representative Chinese-Prevalent Strain of Avian Nephritis Virus in Healthy Chicken Flocks
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Abstract

Avian nephritis virus (ANV), which belongs to the Astroviridae family, has been associated with acute nephritis in chickens. Cases of ANV infection have been recorded in Japan and in several European countries. However, related studies have never been performed in China. Thus, this study isolated ANV in Chinese chicken flocks. ANV RNA was detected by reverse transcription-PCR in stool samples collected from healthy layer chickens in the Sichuan Province of China in 2009. Of the 192 stool specimens collected, 32.3% (62/192) were positive for ANV infection. The whole genome of ANV-Sichuan54, the first representative Chinese strain, was 6941 nudeotides in length, including the 5' untranslated region, three open reading frames (ORFs), a 3' UTR, and a poly-(A) tail. Comparative and phylogenetic analyses based on partial RNA-dependent RNA polymerase (ORFlb) demonstrated that the majority of ANV investigations were more closely related to the U. S. ANV strain (DQ324827-324836) than to the G-4260 (AB033998). El virus de la nefritis aviar (ANV), que pertenece a la família Astroviridae, se ha asociado con nefritis aguda en pollos. Casos de la infección por el virus de la nefritis aviar ANV se han registrado en Japón y en vários países europeos. Sin embargo, estudios relacionados no se han llevado a cabo en China. De esta manera, en este estudio se aisló el virus de la nefritis aviar en parvadas de pollos de China. Se detectó el ARN de este virus mediante la transcripción reversa y PCR en muestras de excretas de gallinas ponedoras sanas en la provincia de Sichuan en China en 2009. De las 192 muestras de heces recogidas, el 32.3% (62/192) fueron positivas para la infección por el virus de la nefritis aviar. El genoma completo del virus de la nefritis infecciosa Sichuan54, la primera cepa representativa de China tenia 6,941 nucleótidos de longitud, incluyendo la región sin traducir, tres marcos de lectura continua (ORF),una región 3' UTR 5, y una cola de poli-(A). El análisis comparativo y el análisis filogenètico basado en la información parcial de la polimerasa de ARN dependiente de ARN (marco de lectura lb) demostraron en la mayoria de las investigaciones que el virus de la nefritis aviar estaba más estrechamente relacionado con la cepa de los Estados Unidos (DQ324827-324836) en comparación con la cepa G-4260 (AB033998).

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