Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Highly Purified CD25- Resting T Cells Cannot be Infected de novo with HIV-1

Chin-Sheng Chou, Octavio Ramilo and Ellen S. Vitetta
Proceedings of the National Academy of Sciences of the United States of America
Vol. 94, No. 4 (Feb. 18, 1997), pp. 1361-1365
Stable URL: http://www.jstor.org/stable/41336
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Highly Purified CD25- Resting T Cells Cannot be Infected de novo with HIV-1
Preview not available

Abstract

Previous studies have demonstrated that the expression of CD25 can distinguish CD25- latently infected cells from CD25+ cells actively producing virus. Our studies were designed to characterize the nature and stability of the viral genome in CD25- quiescent HIV-1-infected cells and to determine whether these cells could be infected de novo with HIV-1. Our results show that: (i) When unfractionated peripheral blood mononuclear cells are first infected with HIV-1 and the CD25- cells then isolated, the latter contain only incomplete DNA transcripts and no full-length DNA or 2-LTR circles. Phytohemagglutinin activation of these CD25- cells results in the generation of full-length viral DNA and p24 production. (ii) When CD25- CD4+ cells are first purified from peripheral blood mononuclear cells and then incubated with HIV-1, viral DNA cannot be detected, suggesting that these purified cells cannot be infected. Furthermore, CD25- adherent cells do not facilitate the infection of CD4+ CD25- T cells when they were present at the time of incubation with HIV-1. Taken together, these studies suggest either that (i) the CD25- cells containing incomplete DNA transcripts are derived from infected-activated CD25+ cells, which subsequently become CD25- or (ii) the presence of CD25+ cells is required for the infection of CD25- cells in vitro.

Page Thumbnails

  • Thumbnail: Page 
1361
    1361
  • Thumbnail: Page 
1362
    1362
  • Thumbnail: Page 
1363
    1363
  • Thumbnail: Page 
1364
    1364
  • Thumbnail: Page 
1365
    1365