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Highly Purified CD25- Resting T Cells Cannot be Infected de novo with HIV-1
Chin-Sheng Chou, Octavio Ramilo and Ellen S. Vitetta
Proceedings of the National Academy of Sciences of the United States of America
Vol. 94, No. 4 (Feb. 18, 1997), pp. 1361-1365
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/41336
Page Count: 5
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Previous studies have demonstrated that the expression of CD25 can distinguish CD25- latently infected cells from CD25+ cells actively producing virus. Our studies were designed to characterize the nature and stability of the viral genome in CD25- quiescent HIV-1-infected cells and to determine whether these cells could be infected de novo with HIV-1. Our results show that: (i) When unfractionated peripheral blood mononuclear cells are first infected with HIV-1 and the CD25- cells then isolated, the latter contain only incomplete DNA transcripts and no full-length DNA or 2-LTR circles. Phytohemagglutinin activation of these CD25- cells results in the generation of full-length viral DNA and p24 production. (ii) When CD25- CD4+ cells are first purified from peripheral blood mononuclear cells and then incubated with HIV-1, viral DNA cannot be detected, suggesting that these purified cells cannot be infected. Furthermore, CD25- adherent cells do not facilitate the infection of CD4+ CD25- T cells when they were present at the time of incubation with HIV-1. Taken together, these studies suggest either that (i) the CD25- cells containing incomplete DNA transcripts are derived from infected-activated CD25+ cells, which subsequently become CD25- or (ii) the presence of CD25+ cells is required for the infection of CD25- cells in vitro.
Proceedings of the National Academy of Sciences of the United States of America © 1997 National Academy of Sciences