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Role of Protein Kinase C, G-Protein Coupled Receptors, and Calcium Flux during Metamorphosis of the Sea Urchin Strongylocentrotus purpuratus

G. Amador-Cano, E. Carpizo-Ituarte and D. Cristino-Jorge
Biological Bulletin
Vol. 210, No. 2 (Apr., 2006), pp. 121-131
DOI: 10.2307/4134601
Stable URL: http://www.jstor.org/stable/4134601
Page Count: 11
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Role of Protein Kinase C, G-Protein Coupled Receptors, and Calcium Flux during Metamorphosis of the Sea Urchin Strongylocentrotus purpuratus
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Abstract

Artificial inducers have been used to study signal-transduction pathways involved in metamorphosis of some marine invertebrates. However, the transduction mechanisms for echinoderms have been less explored. In the present study, participation of protein kinase C (PKC), G-protein-coupled receptors (GPCRs), and calcium has been investigated during metamorphosis of the sea urchin Stronglylocentrotus purpuratus. Competent larvae were induced with different drugs that activate (PKC and GP activators, Ca2+ ionophores, and inhibitors of Ca2+ ATPase) or inhibit (PKC, G-protein, and Ca2+ flux inhibitors) metamorphosis. Six of the compounds were effective: the PKC activators TPA and indolactam; the G-protein inhibitors suramin and guanosine; the calcium ionophore A23187, and the calcium ATPase inhibitor thapsigargin. TPA was effective at $0.001 \mu M$; indolactam was effective at $0.001 \mu M$. In the presence of KCl as inducer, the G-protein inhibitor suramin was effective at 10 μ M and guanosine at $0.001 \mu M$. In the presence of a bacterial film as inducer, suramin was effective at 50 μ M, and guanosine inhibited metamorphosis at 1 μ M. A23187 was effective at $5 and 10 \mu M$ and thapsigargin at $50 and 100 \mu M$. Our results indicate that GPCRs, protein kinase C, and calcium participate in the metamorphosis of S. purpuratus. These elements of the transduction pathways triggered during metamorphosis may be part of a cascade of signal transduction routes that interact from induction to the end of the morphogenetic events that shape the postlarval form. In addition, according to the results obtained with G-protein inhibitors, the GPCRs may be shared between the artificial (KCl) and natural (biofilm) inducers.

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