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Drosophila CENH3 Is Sufficient for Centromere Formation
Maria José Mendiburo, Jan Padeken, Stefanie Fülöp, Aloys Schepers and Patrick Heun
New Series, Vol. 334, No. 6056 (4 November 2011), pp. 686-690
Published by: American Association for the Advancement of Science
Stable URL: http://www.jstor.org/stable/41351648
Page Count: 5
You can always find the topics here!Topics: Centromeres, Plasmids, Kinetochores, Chromosomes, Cell lines, Epigenetics, Chromatin, Drosophila, Histones, Nucleosomes
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CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity. To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-Lacl fusion protein to stably integrated lac operator (lacO) arrays. This ectopic CID focus assembles a functional kinetochore and directs incorporation of CID molecules without the Lad-anchor, providing evidence for the self-propagation of the epigenetic mark. CID-GFP-Lacl-bound extrachromosomal lacO plasmids can assemble kinetochore proteins and bind microtubules, resulting in their stable transmission for several cell generations even after eliminating CID-GFP-Lacl. We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function.
Science © 2011 American Association for the Advancement of Science