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PERSISTENT TAPETAL CELL1 Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice

Hui Li, Zheng Yuan, Gema Vizcay-Barrena, Caiyun Yang, Wanqi Liang, Jie Zong, Zoe A. Wilson and Dabing Zhang
Plant Physiology
Vol. 156, No. 2 (June 2011), pp. 615-630
Stable URL: http://www.jstor.org/stable/41434330
Page Count: 16
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
PERSISTENT TAPETAL CELL1 Encodes a PHD-Finger Protein That Is Required for Tapetal Cell Death and Pollen Development in Rice
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Abstract

In higher plants, timely degradation of tapetai cells, the innermost sporophytic cells of the anther wall layer, is a prerequisite for the development of viable pollen grains. However, relatively little is known about the mechanism underlying programmed tapetai cell development and degradation. Here, we report a key regulator in monocot rice (Oryza sauva), PERSISTANT TAPETAL CELLI (PTCl), which controls programmed tapetal development and functional pollen formation. The evolutionary significance of PTCl was revealed by partial genetic complementation of the homologous mutation MALE STERILITY1 (MSI) in the dicot Arabidopsis (Arabidopsis thaliana). PTCl encodes a PHD-finger (for plant homeodomain) protein, which is expressed specifically in tapetai cells and microspores during anther development in stages 8 and 9, when the wild-type tapetai cells initiate a typical apoptosis-like cell death. Even though ptcl mutants show phenotypic similarity to msl in a lack of tapetai DNA fragmentation, delayed tapetai degeneration, as well as abnormal pollen wall formation and aborted microspore development, the ptcl mutant displays a previously unreported phenotype of uncontrolled tapetai proliferation and subsequent commencement of necrosis-like tapetai death. Microarray analysis indicated that 2,417 tapetum-and microspore-expressed genes, which are principally associated with tapetai development, degeneration, and pollen wall formation, had changed expression in ptcl anthers. Moreover, the regulatory role of PTCl in anther development was revealed by comparison with MS2 and other rice anther developmental regulators. These findings suggest a diversified and conserved switch of PTCl /MSI in regulating programmed male reproductive development in both dicots and monocots, which provides new insights in plant anther development.

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