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RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities
Amy K. Barczak, James E. Gomez, Benjamin B. Kaufmann, Ella R. Hinson, Lisa Cosimi, Mark L. Borowsky, Andrew B. Onderdonk, Sarah A. Stanley, Devinder Kaur, Kevin F. Bryant, David M. Knipe, Alexander Sloutsky and Deborah T. Hung
Proceedings of the National Academy of Sciences of the United States of America
Vol. 109, No. 16 (April 17, 2012), pp. 6217-6222
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/41588500
Page Count: 6
You can always find the topics here!Topics: Antibiotics, Signatures, Pathogens, RNA, Urine, Mycobacterium tuberculosis, Specimens, Genes, Resistance mechanisms, Blood
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With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to diagnosing the presence and drug-susceptibility of infectious diseases based on direct detection of RNA from clinical samples. We demonstrate that species-specific RNA signatures can be used to identify a broad spectrum of infectious agents, including bacteria, viruses, yeast, and parasites. Moreover, we show that the behavior of a small set of bacterial transcripts after a brief antibiotic pulse can rapidly differentiate drug-susceptible and -resistant organisms and that these measurements can be made directly from clinical materials. Thus, transcriptional signatures could form the basis of a uniform diagnostic platform applicable across a broad range of infectious agents.
Proceedings of the National Academy of Sciences of the United States of America © 2012 National Academy of Sciences