You are not currently logged in.
Access your personal account or get JSTOR access through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Cryo-EM structure of a 3D DNA-origami object
Xiao-chen Bai, Thomas G. Martin, Sjors H. W. Scheres and Hendrik Dietz
Proceedings of the National Academy of Sciences of the United States of America
Vol. 109, No. 49 (December 4, 2012), pp. 20012-20017
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/41830457
Page Count: 6
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNAtemplated design steered by structural feedback.
Proceedings of the National Academy of Sciences of the United States of America © 2012 National Academy of Sciences