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NUCLEOLYTIC ACTIVITIES IN PEPPER LEAVES REACTING HYPERSENSITIVELY TO XANTHOMONAS CAMPESTRIS PV. VESICATORIA

R. Buonaurio, A. Polverari, G. Tarparelli and S. Guiderdone
Journal of Plant Pathology
Vol. 83, No. 2 (July 2001), pp. 119-124
Stable URL: http://www.jstor.org/stable/41998047
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
NUCLEOLYTIC ACTIVITIES IN PEPPER LEAVES REACTING HYPERSENSITIVELY TO XANTHOMONAS CAMPESTRIS PV. VESICATORIA
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Abstract

A substrate-based gel assay was used to determine RNase and DNase activities in the incompatible interaction between pepper leaves (cv. 'Early Calwonder 10R') and the avirulent race 2 of Xanthomonas campestris pv. vesicatoria from 0 to 24 hours after inoculation, and for comparison in leaves inoculated with the virulent race 1 of the same bacterium (compatible interaction). Two major bands of RNase activity, with apparent molecular mass of 18 and 27 kDa were detected in pepper leaf extracts. Both activities were markedly inhibited by Mg²⁺ and Zn²⁺; activity of the 18 kDa RNase was stimulated by Ca²⁺. Activity banding at 25 kDa activity, degrading both RNA and DNA as substrate (nuclease activity) and inhibited by EDTA and Zn²⁺, was detected in bacterial cell extracts and in culture fluids of both races 1 and 2 of X. campestris pv. vesicatoria. No significant changes in plant nucleolytic activity were detected in hypersensitively reacting leaves, whereas an increase in the bacterial 25 kDa activity band was observed in both compatible and incompatible interactions.

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