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Isolation and Partial Characterization of Soluble Alfalfa Proteins

L. L. Hood and J. R. Brunner
Economic Botany
Vol. 30, No. 3 (Jul. - Sep., 1976), pp. 285-290
Published by: Springer on behalf of New York Botanical Garden Press
Stable URL: http://www.jstor.org/stable/4253746
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Isolation and Partial Characterization of Soluble Alfalfa Proteins
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Abstract

Soluble proteins, representing ≃18% of total plant protein, were extracted from freshly harvested alfalfa. Two fractions based on solubility in one-half saturated $(NH_{4})_{2}SO_{4}-Fraction$ G (insoluble) and Fraction A (soluble)-were obtained, representing two-thirds and one-third of the extracted protein, respectively. Fraction G contained less hexose-like and more lipid substances than Fraction A which was characterized by an unusually high ash content. Gel electrophoretic patterns revealed greater heterogeneity in Fraction A than in Fraction G, whereas the reverse situation was evident in sedimentation-velocity patterns. The three sedimenting boundaries apparent in Fraction G has $S_{20,app}$ values of 29.2 (4%), 17.4 (61%) and 3.2 (35%). The $S_{20,app}$ 3.2 boundary corresponded to the single boundary observed for Fraction A. Moving-boundary electrophoresis revealed a single boundary for each fraction, possessing mobilities of -6.5 (Fraction G) and -7.5 (Fraction A) Tiselius units, respectively.

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