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EJC core component MLN51 interacts with elF3 and activates translation
Pierre-Etienne Chazal, Elisabeth Daguenet, Corinne Wendling, Nathalie Ulryck, Catherine Tomasetto, Bruno Sargueil and Hervé Le Hir
Proceedings of the National Academy of Sciences of the United States of America
Vol. 110, No. 15 (April 9, 2013), pp. 5903-5908
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/42590327
Page Count: 6
You can always find the topics here!Topics: Messenger RNA, Journalism, Splicing, Exons, Antibodies, Proteins, RNA, HEK293 cells, Open reading frames, Small interfering RNA
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The multiprotein exon junction complex (EJC), deposited by the splicing machinery, is an important constituent of messenger ribonucleoprotein particles because it participates to numerous steps of the mRNA lifecycle from splicing to surveillance via nonsense-mediated mRNA decay pathway. By an unknown mechanism, the EJC also stimulates translation efficiency of newly synthesized mRNAs. Here, we show that among the four EJC core components, the RNA-binding protein metastatic lymph node 51 (MLN51) is a translation enhancer. Overexpression of MLN51 preferentially increased the translation of intron-containing reporters via the EJC, whereas silencing MLN51 decreased translation. In addition, modulation of the MLN51 level in cell-free translational extracts confirmed its direct role in protein synthesis. Immunoprecipitations indicated that MLN51 associates with translation-initiating factors and ribosomal subunits, and in vitro binding assays revealed that MLN51, alone or as part of the EJC, interacts directly with the pivotal eukaryotic translation initiation factor elF3. Taken together, our data define MLN51 as a translation activator linking the EJC and the translation machinery.
Proceedings of the National Academy of Sciences of the United States of America © 2013 National Academy of Sciences