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Journal Article

Influencia de distintos factores en las posibilidades de propagación "in vitro" de Microcycas calocoma (Miq.) A.DC.

Esperanza Peña García, Emma Grillo Mensa and Dalia Pérez Montesino
Revista del Jardín Botánico Nacional
Vol. 13 (1992), pp. 83-93
Stable URL: http://www.jstor.org/stable/42597452
Page Count: 11
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Influencia de distintos factores en las posibilidades de propagación "in vitro" de Microcycas calocoma (Miq.) A.DC.
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Abstract

Se estudia la influencia de distintos factores en el comportamiento in vitro de esporangios, fragmentos del macrogametofito y posibles primeras fases en el desarrollo de semillas de Microcycas calocoma (Miq.) A.DC. utilizando material colectado en diferentes épocas del año, distintos medios minerales, variaciones en los factores de crecimiento y sus concentraciones, y dos condiciones de iluminación. Se evidenció la relación de cada uno de los factores de variación con el tiempo de formación y características de los callos, la diferenciación y características de las raíces, la especificidad de la acción de factores de crecimiento y la composición mineral de los medios de cultivo en la obtención de callos. Se discuten los resultados. The influence of factors related to in vitro proliferation of seminal primordia, macrogametophyte portions and possible primer stages of seed development of Microcycas calocoma (Miq.) A.DC. were studied. The effect of varying the expiant source (by collecting material at different seasons), different mineraf media, variation in types and concentration of growth factors, in addition to the application of two ilumination conditions were taken into account. Relations between each source of variation with time for callus initiation and its characteristics, differentiation and types of roots, specificity in growth factor action and mineral composition of media for calli obtention were evident. Results are discussed.

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