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Journal Article

The Carotenoid Hydrocarbons of Euglena gracilis and Derived Mutants

J. A. Gross, Richard J. Stroz and G. Britton
Plant Physiology
Vol. 55, No. 2 (Feb., 1975), pp. 175-177
Stable URL: http://www.jstor.org/stable/4263894
Page Count: 3
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The Carotenoid Hydrocarbons of Euglena gracilis and Derived Mutants
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Abstract

An examination of the carotene fractions extracted from Euglena gracilis Z and pressure-bleached Euglena mutants PR-1, PR-2, PR-3, and PR-4 revealed phytoene in mutants PR-1, PR-2, and PR-3. Photosynthetic E. gracilis Z cultured at different light intensities showed no detectable phytoene, nor was phytoene found in mutant PR-4. However, dark-cultured E. gracilis Z yielded readily assayable amounts of phytoene. With the exception of PR-4, in which no $\text{C}_{40}$ carotenoids were detected, the following carotenes were identified in all from their mass spectra: phytoene, phytofluene, ζ-carotene, β-zeacarotene, and β-carotene. Of these, phytoene and β-zeacarotene had not previously been unequivocally identified in Euglena.

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