You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The Occurrence and Development of Amylase Enzymes in Incubated, De-Embryonated Maize Kernels
L. D. Goldstein and Paul H. Jennings
Vol. 55, No. 5 (May, 1975), pp. 893-898
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/4264039
Page Count: 6
You can always find the topics here!Topics: Enzymes, Incubation, Corn, Endosperm, Embryos, Gels, Barley, Electrophoresis, Starches, Reaction products
Were these topics helpful?See somethings inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
The development of amylase activity in extracts from de-embryonated and GA3-treated de-embryonated maize kernels (Zea mays L.) was determined during a 10-day incubation period. The increase in activity was compared with activity extracted from endosperms dissected from germinating whole kernels. Chromatographic analysis of reaction products as well as physicochemical characterization demonstrated that the activities from GA3-treated and nontreated tissue were comparable and that part of the activity was attributable to α-amylase. Concomitant with the increase in activity was the appearance of a number of starch-degrading bands, as evidenced by poly-acrylamide gel electrophoresis. Actinomycin-D (20 μg/ml) and cycloheximide (5 μg/ml), when present in the incubation medium at early periods of incubation, were capable of inhibiting the development of amylase activity and of preventing the appearance of the starch-degrading bands. The results indicate that the development of α-amylase activity in de-embryonated maize kernels is independent of an embryo or an exogenous source of gibberellic acid and suggest that this process involved protein synthesis.
Plant Physiology © 1975 American Society of Plant Biologists (ASPB)