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Incorporation of Cytokinin $\text{N}^{6}$-Benzyladenine into Tobacco Callus Transfer Ribonucleic Acid and Ribosomal Ribonucleic Acid Preparations

Donald J. Armstrong, Norimoto Murai, Barbara J. Taller and Folke Skoog
Plant Physiology
Vol. 57, No. 1 (Jan., 1976), pp. 15-22
Stable URL: http://www.jstor.org/stable/4264280
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Incorporation of Cytokinin $\text{N}^{6}$-Benzyladenine into Tobacco Callus Transfer Ribonucleic Acid and Ribosomal Ribonucleic Acid Preparations
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Abstract

The incorporation of the cytokinin $\text{N}^{6}$-benzyladenine into tobacco (Nicotiana tabacum) callus tRNA and rRNA preparations isolated from tissue grown on medium containing either $\text{N}^{6}$-benzyladenine-8-14C or $\text{N}^{6}$-benzyladenine-8-14C: benzene-3H(G) has been examined. $\text{N}^{6}$-benzyladenine was incorporated into both the tRNA and rRNA preparations as the intact base. Over 90% of the radioactive $\text{N}^{6}$-benzyladenosine recovered from the RNA preparations was associated with the rRNA. Purification of the crude rRNA by either MAK chromatography or Sephadex G-200 gel filtration had no effect on the $\text{N}^{6}$-benzyladenosine content of the RNA preparation. The distribution of $\text{N}^{6}$-benzyladenosine moieties in tobacco callus tRNA fractionated by BD-cellulose chromatography did not correspond to the distribution of ribosylzeatin activity. $\text{N}^{6}$-benzyladenosine was released from the rRNA preparation by treatment with venom phosphodiesterase and phosphatase, ribonuclease T2 and phosphatase, or ribonuclease T2 and a 3′-nucleotidase. $\text{N}^{6}$-benzyladenosine was not released from the RNA preparation by treatment with either ribonuclease T2 or phosphatase alone or by successive treatment with ribonuclease T2 and a 5′-nucleotidase. Brief treatment of the rRNA preparation with ribonuclease T1 and pancreatic ribonuclease converted the $\text{N}^{6}$-benzyladenosine moieties into an ethyl alcohol soluble form. On the basis of these and earlier results, the $\text{N}^{6}$-benzyladenosine recovered from the tobacco callus RNA preparations appears to be present as a constituent of RNA and not as a nonpolynucleotide contaminant.

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