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A Comparison of Glutamate Synthase Obtained from Maize Endosperms and Roots

Ann Oaks, Karen Jones and Santosh Misra
Plant Physiology
Vol. 63, No. 5 (May, 1979), pp. 793-795
Stable URL: http://www.jstor.org/stable/4265747
Page Count: 3
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
A Comparison of Glutamate Synthase Obtained from Maize Endosperms and Roots
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Abstract

Glutamate synthase (EC 2.6.1.53) has been examined in developing endosperms and roots of maize. KCl is required for maximum activity in each tissue. The effect with KCl is seen with buffer strength of 25 to 100 millimolar in the assay. The optimum concentration for the enzyme from endosperm is 20 millimolar and for the enzyme from root tissue the saturating concentration is about 20 millimolar. In root material the enzyme is labile but activity can be restored if KCl is added to the assay. Divalent cations such as Mg2+ or Mn2+ also activate the enzyme to some extent. In each case NADH or NADPH can serve as reductant. The reaction is insensitive to α-aminooxyacetate, but is inhibited by glutamate, the glutamate analogs methionine sulfoximine and methionine sulfone, and by the glutamine analogs azaserine and albizziin.

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