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Characteristics of Teratomas Regenerated in vitro from Octopine-Type Crown Gall
Lowell D. Owens
Vol. 69, No. 1 (Jan., 1982), pp. 37-40
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/4267140
Page Count: 4
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Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. Detached leaves from T5 shoots also synthesized octopine. These results establish that shoots from this octopine-type tumor contain transformed cells and are true crown-gall teratomas.
Plant Physiology © 1982 American Society of Plant Biologists (ASPB)