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Characteristics of Teratomas Regenerated in vitro from Octopine-Type Crown Gall

Lowell D. Owens
Plant Physiology
Vol. 69, No. 1 (Jan., 1982), pp. 37-40
Stable URL: http://www.jstor.org/stable/4267140
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Characteristics of Teratomas Regenerated in vitro from Octopine-Type Crown Gall
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Abstract

Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. Detached leaves from T5 shoots also synthesized octopine. These results establish that shoots from this octopine-type tumor contain transformed cells and are true crown-gall teratomas.

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