Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Oxidation of Reduced Pyridine Nucleotide by a System Using Ascorbate and Hydrogen Peroxide from Plants and Algae

Yoke Wah Kow, Douglas A. Smyth and Martin Gibbs
Plant Physiology
Vol. 69, No. 1 (Jan., 1982), pp. 72-76
Stable URL: http://www.jstor.org/stable/4267147
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Oxidation of Reduced Pyridine Nucleotide by a System Using Ascorbate and Hydrogen Peroxide from Plants and Algae
Preview not available

Abstract

A NAD(P)H oxidizing system (NAAP) was detected and partially purified from leaves of spinach and Sedum praealtum, seeds and leaves of pea and cells of green and red algae which oxidized NAD(P)H in the presence of ascorbate and H2O2. The partially-purified spinach system had substrate Km values of 5 micromolar for NADH, 50 micromolar for H2O2, and 300 micromolar for L-ascorbic acid at the pH optimum of 6.8. NADH was a better electron donor than NADPH. Among other electron donors, isoascorbic acid had considerable activity but hydroquinone and resorcinol had only weak activities. The enzyme was inhibited by cyanide, α,α′-dipyridyl, and mono-and di-thiol reagents. Inhibition by thiol-reagents was partially restored by Fe2+ as was enzymic activity lost following dialysis against buffer. Subcellular localization studies with spinach and S. praealtum leaves indicated that a portion of the cell's NAAP was in the chloroplast fraction. Photosynthetic conditions resulted in a decrease in this activity solubilized from spinach and S. praealtum chloroplasts. The presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea or Fe2+ in the incubation medium eliminated the light-mediated inhibition of NAAP. NAAP may function in the recycling of NAD(P)H generated in the dark within the chloroplast. Inasmuch as all preparations of NAAP contained ascorbate peroxidase activity, the data do not rule out the possibility that NAAP is the same protein as ascorbate peroxidase or, alternatively, a combination of ascorbate peroxidase and some other enzyme.

Page Thumbnails

  • Thumbnail: Page 
72
    72
  • Thumbnail: Page 
73
    73
  • Thumbnail: Page 
74
    74
  • Thumbnail: Page 
75
    75
  • Thumbnail: Page 
76
    76