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A 31P Nuclear Magnetic Resonance Study of Intracellular pH of Plant Cells Cultivated in Liquid Medium
Jean-Baptiste Martin, Richard Bligny, Fabrice Rebeille, Roland Douce, Jean-Jacques Leguay, Yves Mathieu and Jean Guern
Vol. 70, No. 4 (Oct., 1982), pp. 1156-1161
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/4267657
Page Count: 6
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31P nuclear magnetic resonance has been used to study the vacuolar and cytoplasmic pH of Acer pseudoplatanus, Catharanthus roseus, and Glycine max cells grown as cell suspensions. The adaptation of this technique to plant cells grown in liquid medium is described with emphasis on the removal of Mn2+ and phosphate from the extracellular medium and on providing the O2 supply of the cells in the nuclear magnetic resonance tube and the various problems of calibration. Aerobic and anaerobic cells show large differences in their glucose-6-phosphate, their cytoplasmic inorganic phosphate pools, and their cytoplasmic pH. Differences in the relative sizes of the cytoplasmic and vacuolar inorganic phosphate pools have been observed for the three cell strains studied.
Plant Physiology © 1982 American Society of Plant Biologists (ASPB)