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Characterization of a Selenocystine-Resistant Carrot Cell Line: Alterations in Cystine and Sulfate Uptake
Ian J. Furner and Zinmay R. Sung
Vol. 71, No. 3 (Mar., 1983), pp. 547-550
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/4267883
Page Count: 4
You can always find the topics here!Topics: Sulfates, Cell lines, Plants, Sulfur, Cell growth, Carrots, Cellular metabolism, Cultured cells, Haploidy, Cell culture techniques
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A selenocystine-resistant carrot cell line, C-1, was isolated from a haploid carrot (Daucus carota) cell culture, HA. The C-1 variant takes up cystine, but not cysteine, more slowly than does HA. The selenocystine resistance is maintained in culture in the absence of selection and is expressed in regenerated plants. Results based on chromatographic separation of sulfur metabolites from cells fed with [35S]cystine suggest a block either in the uptake or reduction of cystine in the variant. Both lines can grow on cystine as sole sulfur source. Growth of the HA line on cystine suppressed the development of sulfate uptake capacity (Furner, Sung 1982 Proc Natl Acad Sci USA 79: 1149-1153), while cystine-grown C-1 cells have high levels of sulfate uptake capacity. We suggest that the C-1 line, grown on cystine, accumulates an insufficient quantity of some sulfur metabolite, which is involved in the control of sulfate uptake, to suppress the uptake. C-1 grown on cystine is more sensitive than HA to growth inhibition by the sulfate analog selenate.
Plant Physiology © 1983 American Society of Plant Biologists (ASPB)