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Immunodetection of the Ferredoxin-NADP⁺ Oxidoreductase-Binding Protein Complex in Thylakoids of Different Higher Plant Species
Fernando C. Soncini and Rubén H. Vallejos
Vol. 90, No. 2 (Jun., 1989), pp. 372-375
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/4272085
Page Count: 4
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Monospecific polyclonal antibodies against thylakoid ferredoxin-NADP+ oxidoreductase and its binding protein from Spinacia oleracea were used to detect the presence of these proteins in different higher plants, including C3, C4, and Crassulacean acid metabolism species. A remarkable conservation of antigenic determinants in all the species analyzed was demonstrated for both the reductase and its binding protein. The association of these polypeptides in a complex was detected by immunoprecipitation.
Plant Physiology © 1989 American Society of Plant Biologists (ASPB)