Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Divergent and Conserved Features in the Spatial Expression of the Drosophila pseudoobscura esterase-5B Gene and the esterase-6 gene of Drosophila melanogaster

Natalia A. Tamarina, Michael Z. Ludwig and Rollin C. Richmond
Proceedings of the National Academy of Sciences of the United States of America
Vol. 94, No. 15 (Jul. 22, 1997), pp. 7735-7741
Stable URL: http://www.jstor.org/stable/42750
Page Count: 7
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Divergent and Conserved Features in the Spatial Expression of the Drosophila pseudoobscura esterase-5B Gene and the esterase-6 gene of Drosophila melanogaster
Preview not available

Abstract

The regulatory regions of homologous genes encoding esterase 6 (Est-6) of Drosophila melanogaster and esterase 5B (Est-5B) of Drosophila pseudoobscura show very little similarity. We have undertaken a comparative study of the pattern of expression directed by the Est-5B and Est-6 5′-flanking DNA to attempt to reveal conserved elements regulating tissue-specific expression in adults. Esterase regulatory sequences were linked to a lacZ reporter gene and transformed into D. melanogaster embryos. Est-5B, 5′ upstream elements, give rise to a β -galactosidase expression pattern that coincides with the wild-type expression of Est-5B in D. pseudoobscura. The expression patterns of the Est-5B/lacZ construct are different from those of a fusion gene containing the upstream region of Est-6. Common sites of expression for both kinds of constructs are the third segment of antenna, the maxillary palps, and salivary glands. In vitro deletion mutagenesis has shown that the two genes have a different organization of regulatory elements controlling expression in both the third segment of antenna and maxillary palps. The results suggest that the conservation of the expression pattern in genes that evolved from a common ancester may not be accompanied by preservation of the corresponding cis-regulatory elements.

Page Thumbnails

  • Thumbnail: Page 
7735
    7735
  • Thumbnail: Page 
7736
    7736
  • Thumbnail: Page 
7737
    7737
  • Thumbnail: Page 
7738
    7738
  • Thumbnail: Page 
7739
    7739
  • Thumbnail: Page 
7740
    7740
  • Thumbnail: Page 
7741
    7741