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Genetic Manipulation of the Cyanobacterium Synechocystis sp. PCC 6803: Development of Strains Lacking Photosystem I for the Analysis of Mutations in Photosystem II

Lawrence B. Smart, Neil R. Bowlby, Shawn L. Anderson, Idah Sithole and Lee McIntosh
Plant Physiology
Vol. 104, No. 2 (Feb., 1994), pp. 349-354
Stable URL: http://www.jstor.org/stable/4275628
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Genetic Manipulation of the Cyanobacterium Synechocystis sp. PCC 6803: Development of Strains Lacking Photosystem I for the Analysis of Mutations in Photosystem II
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Abstract

We have taken a genetic approach to eliminating the presence of photosystem I (PSI) in site-directed mutants of photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. By selecting under light-activated heterotrophic conditions, we have inactivated the psaA-psaB operon encoding the PSI reaction center proteins in cells containing deletions of the three psbA genes. We have also introduced deletions into both copies of psbD in a strain containing a mutation that inactivates psaA (ADK9). These strains, designated $\text{D}1^{-}/\text{PSI}^{-}$ and $\text{D}2^{-}/\text{PSI}^{-}$, may serve as recipient strains for the incorporation of site-directed mutations in either psbA2 or psbD1. The characterization of these cells, which lack both PSI and PSII, is described.

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