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Activities of Transaminases, Amylases and Proteases during Endosperm Degradation in Normal and Opaque-2 Zea mays L cv. Maya
ANA MARIA MONTEIRO and J. METIVIER
Annals of Botany
Vol. 52, No. 4 (October 1983), pp. 535-541
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/42757152
Page Count: 7
You can always find the topics here!Topics: Endosperm, Corn, Seedlings, Amino acids, Enzymes, Protein metabolism, Sugars, Mayan culture, Grains, Storage proteins
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Transaminase, amylase and protease activities were compared in seedlings of normal and Opaque-2 (o2) maize. Transaminase activity, greater in normal maize, was highest in the scutellum from which it decreased rapidly in activity from day 2 after imbibition ; only low activity was observed in endosperm and axis tissue. Amylolytic activity, optimal around pH 5, was greater in normal maize at all stages of endosperm degradation. Activity which was low on day 2, rose to a peak on day 6 and declined afterwards. The level of free sugars in the endosperm of normal was higher than in o2 maize, and in both varieties was highly correlated with amylolysis. Protease activity, optimal at pH 3-6, was also greater in normal endosperms and increased up to day 6 and activity was maintained at this level until around day 14. Although the activity of all three enzyme systems examined was greater in normal maize there were no apparent differences in the overall growth of normal and o2 seedlings during this period.
Annals of Botany © 1983 Oxford University Press