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Conditions for the Attachment of Calystegia Cells to Solid Substrates
A. KOLSTER and R. BEIDERBECK
Annals of Botany
Vol. 59, No. 4 (April 1987), pp. 471-473
Published by: Oxford University Press
Stable URL: http://www.jstor.org/stable/42758019
Page Count: 3
You can always find the topics here!Topics: Mesophyll cells, Plant cells, Cultured cells, Chemical suspensions, Liquids, Immobilized cells, Botany, Cell culture techniques, Incubation
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Mechanically isolated mesophyll cells of Calystegia sepium L. incubated in thin layers of non-agitated liquid media readily attach to their substrate (culture vessel). Conditions for this attachment of living plant cells are: a medium consisting of a low concentration of at least one macroelement and one sugar, a pH value between 5 and 7, and a substrate with a hydrophilic character (e. g. glass, polystyrene).
Annals of Botany © 1987 Oxford University Press