Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Cis-Acting Elements Essential for Light Regulation of the Nuclear Gene Encoding the A Subunit of Chloroplast Glyceraldehyde 3-Phosphate Dehydrogenase in Arabidopsis thaliana

Soo-Chul Park, Hawk-Bin Kwon and Ming-Che Shih
Plant Physiology
Vol. 112, No. 4 (Dec., 1996), pp. 1563-1571
Stable URL: http://www.jstor.org/stable/4277476
Page Count: 9
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Cis-Acting Elements Essential for Light Regulation of the Nuclear Gene Encoding the A Subunit of Chloroplast Glyceraldehyde 3-Phosphate Dehydrogenase in Arabidopsis thaliana
Preview not available

Abstract

We report the characterization of cis-acting elements involved in light regulation of the nuclear gene (GapA) that encodes the A subunit of glyceraldehyde 3-phosphate dehydrogenase in Arabidopsis thaliana. Our previous deletion analyses indicate that the -277 to -195 upstream region of GapA is essential for light induction of the β-glucuronidase reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This region contains three direct repeats with the consensus sequence 5′-CAAATGAA(A/G)A-3′ (Gap boxes). Our results show that 2-bp substitutions of the last four nucleotides (AA or GA) of the Gap boxes by CC abolish light induction of the β-glucuronidase reporter gene in vivo and affect binding of the Gap box binding factor in vitro. We have also identified an additional cis-acting element, AE (Activation Element) box, that is involved in regulation of GapA. A combination of a Gap box trimer and an AE box dimer can confer light responsiveness on the cauliflower mosaic virus 35S promoter containing the -92 to +6 upstream sequence, whereas oligomers of Gap boxes or AE boxes alone cannot confer light responsiveness on the same promoter. These results suggest that Gap boxes and AE boxes function together as the light-responsive element of GapA.

Page Thumbnails

  • Thumbnail: Page 
1563
    1563
  • Thumbnail: Page 
1564
    1564
  • Thumbnail: Page 
1565
    1565
  • Thumbnail: Page 
1566
    1566
  • Thumbnail: Page 
1567
    1567
  • Thumbnail: Page 
1568
    1568
  • Thumbnail: Page 
1569
    1569
  • Thumbnail: Page 
1570
    1570
  • Thumbnail: Page 
1571
    1571