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STA11, a Chlamydomonas reinhardtii Locus Required for Normal Starch Granule Biogenesis, Encodes Disproportionating Enzyme. Further Evidence for a Function of α-1,4 Glucanotransferases during Starch Granule Biosynthesis in Green Algae
Fabrice Wattebled, Jean-Philippe Ral, David Dauvillée, Alan M. Myers, Martha G. James, Ralf Schlichting, Christoph Giersch, Steven G. Ball and Christophe D'Hulst
Vol. 132, No. 1 (May, 2003), pp. 137-145
Published by: American Society of Plant Biologists (ASPB)
Stable URL: http://www.jstor.org/stable/4281082
Page Count: 9
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In Chlamydomonas reinhardtii, the presence of a defective STA11 locus results in significantly reduced granular starch deposition displaying major modifications in shape and structure. This defect simultaneously leads to the accumulation of linear malto-oligosaccharides (MOS). The mutants of STA11 were showed to lack D-enzyme, a plant α-1,4 glucanotransferase analogous to the Escherichia coli amylomaltase. We have cloned and characterized both the cDNA and gDNA corresponding to the C. reinhardtii D-enzyme. We now report allele-specific modifications of the D-enzyme gene in the mutants of STA11. These allele-specific modifications cosegregate with the corresponding sta11 mutations, thereby demonstrating that STA11 encodes D-enzyme. MOS production and starch accumulation were investigated during day and night cycles in wild-type and mutant C. reinhardtii cells. We demonstrate that in the algae MOS are produced during starch biosynthesis and degraded during the phases of net polysaccharide catabolism.
Plant Physiology © 2003 American Society of Plant Biologists (ASPB)