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Growth and Tissue Formation from Single Geranium Cells from Virus-Infected Geraniums
U. Kant and A. C. Hildebrandt
Vol. 7, No. 1 (Jul. - Aug., 1971), pp. 17-20
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/4291577
Page Count: 4
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Single geranium (Pelargonium hortorum, Bailey) cells from callus isolated originally from stem tips of virus-infected plants were grown in microculture chambers in liquid Murashige and Skoog medium supplemented with 0.1 mg per liter of α-naphthalene acetic acid and 10.0 mg per liter of kinetin. Four of 1,000 of these single cells divided in the microculture chamber and produced colonies of 15 to 20 cells in 9 to 16 days. In all of the cases the plane of the first few cell divisions was at right angles to the long axis of the cells. Subsequently, one of the masses of cells obtained from a single cell, when transferred to solid Murashige and Skoog medium, established itself as a clone of callus tissue. Although the yield was low, the results were encouraging for the ultimate production of plants. These results suggested that, just as virus-free tobacco plants have been induced from single cell clones from certain tobacco species, virus-free single geranium cells may be useful to establish single cell clones from which pathogen-free geranium plants may be induced to differentiate.
In Vitro © 1971 Society for In Vitro Biology