Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Characterization of the Transposition Pattern of the Ac Element in Arabidopsis thaliana Using Endonuclease I-SceI

Chiyoko Machida, Hitoshi Onouchi, Jun Koizumi, Susumu Hamada, Endang Semiarti, Satomi Torikai and Yasunori Machida
Proceedings of the National Academy of Sciences of the United States of America
Vol. 94, No. 16 (Aug. 5, 1997), pp. 8675-8680
Stable URL: http://www.jstor.org/stable/42916
Page Count: 6
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Characterization of the Transposition Pattern of the Ac Element in Arabidopsis thaliana Using Endonuclease I-SceI
Preview not available

Abstract

We have investigated physical distances and directions of transposition of the maize transposable element Ac in Arabidopsis thaliana. We prepared a transferred DNA (T-DNA) construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-SceI (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. Three transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. These transgenic plants were crossed with the Arabidopsis that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with endonuclease I-SceI, sizes of segment of DNA were determined by pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results showed that 50% of all transposition events had occurred within 1,700 kb on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites.

Page Thumbnails

  • Thumbnail: Page 
8675
    8675
  • Thumbnail: Page 
8676
    8676
  • Thumbnail: Page 
8677
    8677
  • Thumbnail: Page 
8678
    8678
  • Thumbnail: Page 
8679
    8679
  • Thumbnail: Page 
8680
    8680