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Mycoplasma in African Green Monkey Kidney Cell Cultures: Biochemical Detection and Effects in Virus-Infected Cells

F. van Roy and W. Fiers
In Vitro
Vol. 13, No. 6 (Jun., 1977), pp. 357-365
Stable URL: http://www.jstor.org/stable/4291942
Page Count: 9
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Mycoplasma in African Green Monkey Kidney Cell Cultures: Biochemical Detection and Effects in Virus-Infected Cells
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Abstract

Among a number of techniques for the detection of mycoplasmal contamination in African green monkey kidney (AGMK) cell lines, the assay of uridine phosphorylase activity is unsuitable because of the presence of high levels of endogenous enzymatic activity. A thymidine phosphorylase test, however, based on the Chromatographic analysis of radiolabeled thymidine breakdown, turned out to be a simple and sensitive mycoplasma detection method. We found, using the latter technique, that 0.22-µm-filtered virus inocula could still transfer mycoplasma unless treated with diethyl ether. The effect of mycoplasmal contamination on the synthesis of simian virus 40 and adenovirus in AGMK cells was negligible under the conditions used (no depletion of arginine). Incorporation of radioactive thymidine in viral macromolecules, however, was inhibited severely by the presence of mycoplasma.

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