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Organ Culture of Adult Mouse Intestine: II. Mitotic Activity, DNA Synthesis and Cellular Migration after 24 and 48 Hours of Culture

S. Ferland and J. S. Hugon
In Vitro
Vol. 15, No. 4 (Apr., 1979), pp. 288-293
Stable URL: http://www.jstor.org/stable/4292201
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Organ Culture of Adult Mouse Intestine: II. Mitotic Activity, DNA Synthesis and Cellular Migration after 24 and 48 Hours of Culture
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Abstract

DNA synthesis, mitotic activity and cell migration have been measured in organ culture of adult mouse jejunum during the first 48 hr. Explants cultured in DMEM-HEPES-NCTC-135 medium present a sharp decrease of mitotic activity in the first hours of culture, but mitoses are restored to 80% of the controls between 24 and 48 hr. DNA synthesis follows the same pattern. Cell migration continues during culture.

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