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A Simple Method for in vitro Studies with Rodent Urinary Bladders
C. D. Jackson and Glenn Newport
Vol. 17, No. 2 (Feb., 1981), pp. 107-113
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/4292479
Page Count: 7
You can always find the topics here!Topics: Bladder, Incubation, Urothelium, Urinary bladder, Urine, Epithelial cells, Rodents, Biochemistry, Rats, Histology
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A method was developed for the in vitro study of rodent urinary bladders. The method consists of everting and distending the urinary bladder in a manner to allow exposure of the luminal surface of the urothelium during in vitro incubation while maintaining the integrity of the structure and morphology of the bladder. A technique for selectively removing the urothelium with SDS buffer for biochemical analysis was described. Incorporation of $[^3H]leucine$ into urothelial protein was linear over a 4 h period in the presence of tissue culture medium, but no significant incorporation occurred when urine was used as incubation medium. Autoradiography indicated the $[^3H]leucine$ incorporation was almost exclusively in the urothelial cells with essentially no incorporation by cells below the tunica propria.
In Vitro © 1981 Society for In Vitro Biology