You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Effect of 2,4-Dichlorophenoxyacetic Acid Concentration on Somatic Embryogenesis and Heritable Variation in Soybean [Glycine max (L) Merr.]
Randy C. Shoemaker, Laurie A. Amberger, Reid G. Palmer, Lynnea Oglesby and Jerome P. Ranch
In Vitro Cellular & Developmental Biology. Plant
Vol. 27P, No. 2 (Apr., 1991), pp. 84-88
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/4292932
Page Count: 5
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
The frequency and quality of embryogenic response from cotyledons of immature zygotic soybean embryos varied with 2,4-dichlorophenoxyacetic acid (2,4-D) concentration in the culture medium. The frequency of variants among progeny of regenerated plants decreased with an increase of 2,4-D concentration. Teratogenic effects on embryo morphology and development were greatest at 22.5 µM 2,4-D and decreased with increasing 2,4-D. At the lowest 2,4-D concentration tested, 22.5 µM, morphologically abnormal, cotyledonary-stage somatic embryos were produced. Ten percent or less of these embryos converted to plants. Over the nine genotypes tested, 40% of the families derived from plants regenerated under a low 2,4-D concentration manifested heritable variation. In contrast, embryogeny was suppressed at the globular stage by the highest 2,4-D concentration tested, 200 µM. Eighty to one-hundred percent of the embryos organized under this latter 2,4-D level converted to plants. Only 3% of the families from the progeny of plants regenerated under a high 2,4-D concentration exhibited heritable variation.
In Vitro Cellular & Developmental Biology. Plant © 1991 Society for In Vitro Biology