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Effect of 2,4-Dichlorophenoxyacetic Acid Concentration on Somatic Embryogenesis and Heritable Variation in Soybean [Glycine max (L) Merr.]

Randy C. Shoemaker, Laurie A. Amberger, Reid G. Palmer, Lynnea Oglesby and Jerome P. Ranch
In Vitro Cellular & Developmental Biology. Plant
Vol. 27P, No. 2 (Apr., 1991), pp. 84-88
Stable URL: http://www.jstor.org/stable/4292932
Page Count: 5
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Effect of 2,4-Dichlorophenoxyacetic Acid Concentration on Somatic Embryogenesis and Heritable Variation in Soybean [Glycine max (L) Merr.]
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Abstract

The frequency and quality of embryogenic response from cotyledons of immature zygotic soybean embryos varied with 2,4-dichlorophenoxyacetic acid (2,4-D) concentration in the culture medium. The frequency of variants among progeny of regenerated plants decreased with an increase of 2,4-D concentration. Teratogenic effects on embryo morphology and development were greatest at 22.5 µM 2,4-D and decreased with increasing 2,4-D. At the lowest 2,4-D concentration tested, 22.5 µM, morphologically abnormal, cotyledonary-stage somatic embryos were produced. Ten percent or less of these embryos converted to plants. Over the nine genotypes tested, 40% of the families derived from plants regenerated under a low 2,4-D concentration manifested heritable variation. In contrast, embryogeny was suppressed at the globular stage by the highest 2,4-D concentration tested, 200 µM. Eighty to one-hundred percent of the embryos organized under this latter 2,4-D level converted to plants. Only 3% of the families from the progeny of plants regenerated under a high 2,4-D concentration exhibited heritable variation.

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