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Influence of Ovule Perforation, Plant Growth Regulators, and L-Glutamine on in vitro Growth of Immature Peach Embryos

Alberto C. Q. Pinto, David H. Byrne and Suzanne M. Dethier Rogers
In Vitro Cellular & Developmental Biology. Plant
Vol. 29P, No. 2 (Apr., 1993), pp. 55-58
Stable URL: http://www.jstor.org/stable/4292972
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Influence of Ovule Perforation, Plant Growth Regulators, and L-Glutamine on in vitro Growth of Immature Peach Embryos
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Abstract

Ovule perforation technique and media components (plant growth regulators and L-glutamine) were tested on in vitro growth of immature (<3 mm) embryos of "Springerest" and "Earligrande" peaches. Ovule perforation was 2 to 4 times more effective in promoting embryo growth than leaving ovules intact. L-Glutamine $(400 mg \cdot liter^{-1})$ promoted an increase in growth but could not be used with indole-acetic acid plus kinetin because an antagonistic effect on embryo growth occurred. The use of these exogenous plant growth regulators did not increase embryo growth over in vivo growth.

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