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In vitro Propagation of Cereus peruvianus Mill. (Cactaceae)

Sandra Aparecida de Oliveira, Maria de Fátima Pires da Silva Machado, Alberto José Prioli and Claudete Aparecida Mangolin
In Vitro Cellular & Developmental Biology. Plant
Vol. 31, No. 1 (Jan., 1995), pp. 47-50
Stable URL: http://www.jstor.org/stable/4293055
Page Count: 4
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In vitro Propagation of Cereus peruvianus Mill. (Cactaceae)
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Abstract

Cereus peruvianus seedlings were used as a source of stem expiants to determine the effective conditions for inducing and maintaining callus tissues in a state of rapid growth, as well as to obtain plants regenerated from callus cultures. Factorial combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin in MS medium were tested, and we concluded that the 18.1 µM 2,4-D and 18.6 or 27.9 µM kinetin combinations were suitable for callus induction. The cactus shoots were produced from the friable callus; root elongation occurred within 2 wk in medium without 2,4-D and with 18.6 µM kinetin. This method can be used to rapidly produce many C. peruvianus plants.

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