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Organogenesis and Somatic Embryogenesis in Codiaeum Variegatum (L.) Blume cv. "Corazón de Oro"

Patricia Laura Marconi and Silvia Radice
In Vitro Cellular & Developmental Biology. Plant
Vol. 33, No. 4 (Oct. - Dec., 1997), pp. 258-262
Stable URL: http://www.jstor.org/stable/4293137
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Organogenesis and Somatic Embryogenesis in Codiaeum Variegatum (L.) Blume cv. "Corazón de Oro"
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Abstract

Adventive organogenesis and somatic embryogenesis were induced from leaf explants taken from in vitro or in vivo plants of Codiaeum variegatum cv. "$Coraz\acute{o}$ n de Oro." Shoot multiplication occurred with $N^6-benzyladenine$ (BA) alone, where the simultaneous production of adventitious buds and somatic embryos occurred at the fourth subculture, and on leaves not in contact with the medium. A medium with BA and 2,4 dichlorophenoxy acetic acid (2,4-D) produced the largest organogenic response, for both in vivo- and in vitro-produced explants. Somatic embryogenesis was only induced when such explants were transferred to a medium lacking 2,4-D. Thus, a medium with BA only produced the largest percentage of explants with shoots and embryos. Replacing BA with thidiazuron induced up to 100% bud regeneration on in vitro-produced explants by 60 d, but was slower for in vitro-grown explants. Both types of embryos exhibited growth arrest that was partially overcome by transfer to hormone-free basal medium with activated charcoal. Rooted plants from all explants were successfully obtained on a medium with indole-butyric acid (IBA).

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