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PECTOLYTIC ENZYMES IN INOCULATED AND UNINOCULATED RED CLOVER SEEDLINGS

P. M. BONISH
Plant and Soil
Vol. 39, No. 2 (OCTOBER 1973), pp. 319-328
Published by: Springer
Stable URL: http://www.jstor.org/stable/42932392
Page Count: 10
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PECTOLYTIC ENZYMES IN INOCULATED AND UNINOCULATED RED CLOVER SEEDLINGS
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Abstract

Pectolytic enzymes were studied in inoculated and uninoculated red clover (Trifolium pratense) seedlings grown aseptically in flasks containing distilled water or nitrogen-free salts media. Enzyme activity in root exudates and root extracts depended on the conditions of seedling growth. Tests with sodium polypectate and citrus pectin indicated the presence of two enzymes, specific for pectic acid and pectin respectively. Both enzymes were produced by uninoculated seedlings, and in seedlings inoculated with Rhizobium trifolii, R. leguminosarum or R. lupini, enzyme activity was not correlated with infectivity of the strains.

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