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In Planta Soybean Transformation Technologies Developed in China: Procedure, Confirmation and Field Performance
Ching-Yeh Hu and Lianzheng Wang
In Vitro Cellular & Developmental Biology. Plant
Vol. 35, No. 5 (Sep. - Oct., 1999), pp. 417-420
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/4293277
Page Count: 4
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Soybean is a recalcitrant species for in vitro manipulation. Chinese scientists developed two in planta non-tissue culture soybean transformation procedures: (1) via the "pollen-tube-pathway" to introduce exogenous genomic total DNA of Glycine gracilis, the seed of which consisted of 50% protein, and (2) "ovarian injection" with exogenous plasmid DNA containing atrazine-resistant gene. A high yield and high seed protein (45.44%) cultivar, 'Heisheng 101', resulted from the first method, and atrazine-resistant F1, F2, and F3 plants were obtained from the second method. Both exogenous single-genic Mendelian traits and multi-genic quantitative traits were transferrable with these simple and inexpensive procedures. However, some controversy exists with the acceptance of these novo procedures; mainly because instead of the standard Southern blotting, the RAPD and dot blotting techniques were used in the molecular confirmation of the transgenic status in the reported studies.
In Vitro Cellular & Developmental Biology. Plant © 1999 Society for In Vitro Biology