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In Planta Soybean Transformation Technologies Developed in China: Procedure, Confirmation and Field Performance

Ching-Yeh Hu and Lianzheng Wang
In Vitro Cellular & Developmental Biology. Plant
Vol. 35, No. 5 (Sep. - Oct., 1999), pp. 417-420
Stable URL: http://www.jstor.org/stable/4293277
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
In Planta Soybean Transformation Technologies Developed in China: Procedure, Confirmation and Field Performance
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Abstract

Soybean is a recalcitrant species for in vitro manipulation. Chinese scientists developed two in planta non-tissue culture soybean transformation procedures: (1) via the "pollen-tube-pathway" to introduce exogenous genomic total DNA of Glycine gracilis, the seed of which consisted of 50% protein, and (2) "ovarian injection" with exogenous plasmid DNA containing atrazine-resistant gene. A high yield and high seed protein (45.44%) cultivar, 'Heisheng 101', resulted from the first method, and atrazine-resistant F1, F2, and F3 plants were obtained from the second method. Both exogenous single-genic Mendelian traits and multi-genic quantitative traits were transferrable with these simple and inexpensive procedures. However, some controversy exists with the acceptance of these novo procedures; mainly because instead of the standard Southern blotting, the RAPD and dot blotting techniques were used in the molecular confirmation of the transgenic status in the reported studies.

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