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Effect of Various Growth Regulators on Shoot Regeneration of Sugarcane
K. Chengalrayan and M. Gallo-Meagher
In Vitro Cellular & Developmental Biology. Plant
Vol. 37, No. 4 (Jul. - Aug., 2001), pp. 434-439
Published by: Society for In Vitro Biology
Stable URL: http://www.jstor.org/stable/4293489
Page Count: 6
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Sugarcane (Saccharum spp. hybrid cv. CP 84-1198) embryogenic calluses were induced from young leaves cultured on modified Murashige and Skoog basal medium supplemented with 13.6 µM 2,4-dichlorophenoxyacetic acid. Five concentrations, 0.5, 1.0, 2.5, 5.0, and 10.0 µM, of five different growth regulators, 6-benzylaminopurine, kinetin, 6-γ,γ-(dimethylallylamino)purine, zeatin, and thidiazuron, were tested with or without 22.5 µM α-naphthaleneacetic acid to compare their ability to induce regeneration from embryogenic callus. After 4 wk on medium, the percentage of shoot meristem induction was evaluated, and after 10 wk the total number of shoots produced, as well as the percentage of shoots greater than 1 cm in length, was obtained. Although it had the lowest percentage of elongated shoots, medium containing thidiazuron alone performed better than all other growth regulators tested, with the highest percentage of shoot induction and the largest number of shoots, particularly at a concentration of 2.5 µM.
In Vitro Cellular & Developmental Biology. Plant © 2001 Society for In Vitro Biology