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Organogenesis and Somatic Embryogenesis in Ariocarpus kotschoubeyanus (Lem.) K. Schum. (Cactaceae), an Endemic and Endangered Mexican Species

Katja G. Moebius-Goldammer, Martín Mata-Rosas and Victor M. Chávez-Avila
In Vitro Cellular & Developmental Biology. Plant
Vol. 39, No. 4 (Jul. - Aug., 2003), pp. 388-393
Stable URL: http://www.jstor.org/stable/4293631
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Organogenesis and Somatic Embryogenesis in Ariocarpus kotschoubeyanus (Lem.) K. Schum. (Cactaceae), an Endemic and Endangered Mexican Species
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Abstract

Shoots by direct and indirect organogenesis and somatic embryos were induced from tubercles excised from Ariocarpus kotschoubeyanus seeds germinated in vitro. Shoot formation was greatest (6.3 per explant) when explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BA; $13.3 \mu M$) and $\alpha-naphthaleneacetic$ acid (NAA; $5.4 \mu M$). Individualized shoots were rooted in half-strength MS; the addition of activated charcoal ($1 gl^{-1}$) and the use of sun cap closures to seal containers improved the rooting of shoots. Nearly 20% of the explants produced somatic embryos on media containing combinations of BA ($8.9-22.2 \mu M$) and NAA ($0.5-5.4 \mu M$). The establishment of plantlets in soil presented no significant problems. In vitro culture is a useful option for mass propagation of A. kotschoubeyanus and contributes to its conservation.

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