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In situ nitrate reductase activity as an indicator of nitrate availability

L. PASSAMA, A. GOJON, P. ROBIN and L. SALSAC
Plant and Soil
Vol. 102, No. 1 (1987), pp. 145-148
Published by: Springer
Stable URL: http://www.jstor.org/stable/42936760
Page Count: 4
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In situ nitrate reductase activity as an indicator of nitrate availability
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Abstract

The effect of external nitrate concentration in the nutrient solution on nitrate reduction by 7-day-old barley seedlings was investigated using an in situ nitrate reductase activity (NRA) assay, performed with or without exogenous nitrate during the incubation. The difference between plus and minus nitrate NRA of the leaves related to plus nitrate NRA decreased with increasing nitrate concentration in the nutrient solution. Furthermore, the root contribution to the whole plant NRA became predominant at low external nitrate levels. It is proposed that plus and minus nitrate NRA should be used together as an indicator of nitrate availability in the medium and of root contribution to whole plant nitrate reduction.

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