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Nucleotide sequence and diversity in 16S ribosomal RNA from Frankia

D.E. HARRY, D.C. YANG and J.O. DAWSON
Plant and Soil
Vol. 131, No. 1 (February 1991), pp. 143-146
Published by: Springer
Stable URL: http://www.jstor.org/stable/42936937
Page Count: 4
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Nucleotide sequence and diversity in 16S ribosomal RNA from Frankia
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Abstract

Frankia is the diverse bacterial genus that fixes nitrogen within root nodules of actinorhizal trees and shrubs. Systematic and ecological studies of Frankia have been hindered by the lack of morphological, biochemical, or other markers to readily distinguish strains. Recently, nucleotide sequence of 16 S RNA from the small ribosomal subunit has been used to classify and identify a variety of microorganisms. We report nucleotide sequences from portions of the 16 S ribosomal RNA from Frankia strains Acnll isolated from Alnus viridis ssp. crispa (Ait.) Turrill and Ptll isolated from Purshia tridentata (Pursh) DC. The number of nucleotide base substitutions and gaps we find more than doubles the previously reported sequence diversity for the same variable regions within other strains of Frankia.

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