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Inoculation in vitro of wheat seedlings and wheat straw cultures with Bacillus azotofixans

B.D. DE LIMA and E.G.C. PENIDO
Plant and Soil
Vol. 113, No. 1 (January (I), 1989), pp. 133-136
Published by: Springer
Stable URL: http://www.jstor.org/stable/42937741
Page Count: 4
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Inoculation in vitro of wheat seedlings and wheat straw cultures with Bacillus azotofixans
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Abstract

Bacillus azotofixans is a recently described species capable of fixing molecular nitrogen efficiently. Ecological studies performed in monoxenic wheat cultures, both in 0.7% agar and in vermiculite-sand mixture, showed that no acetylene reduction occurred and that this bacteria did not grow when supplied only with the wheat plant root exudates. However, after glucose addition to the 0.7% agar cultures, acetylene reduction ability (ARA) was detected. Comparing ARA for media with glucose both with and without plants, it was observed that the plants supply some component leading to the increase of the nitrogenase activity, since the ARA doubled in the samples containing plants. In wheat straw cultures a fast growth of the bacteria was observed in the first 24 hours after inoculation, but no acetylene reduction was detected. After glucose addition to the media with and without straw, nitrogenase activity was detected.

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