Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Culture and Characterization of Sinusoidal Endothelial Cells Isolated from Human Liver

George W. Daneker, Serena A. Lund, S. Wright Caughman, Robert A. Swerlick, Andrew H. Fischer, Charles A. Staley and Edwin W. Ades
In Vitro Cellular & Developmental Biology. Animal
Vol. 34, No. 5 (May, 1998), pp. 370-377
Stable URL: http://www.jstor.org/stable/4294782
Page Count: 8
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Culture and Characterization of Sinusoidal Endothelial Cells Isolated from Human Liver
Preview not available

Abstract

Although most vascular models use large vessel endothelial cells from human umbilical veins, there is marked heterogeneity among endothelial cells from different vascular beds and organs. More accurate modeling of endothelial involvement in liver diseases, including metastasis, may result from the use of human hepatic sinusoidal endothelial cells. Liver resection specimens were sectioned, then treated with a 1.2 U/ml dispase solution. The tissue slurry was mechanically disaggregated and separated by centrifugation on a Percoll density gradient. Cells were then cultured in an endothelial-specific media with growth factors. These techniques resulted in a homogeneous monolayer consistent with endothelial cells by light microscopy. An endothelial origin was further confirmed by the expression of Factor VIII, binding of Ulex lectin, and uptake of acetylated low density lipoprotein. Electron microscopy showed transcellular fenestrations consistent with a sinusoidal origin. These human hepatic sinusoidal endothelial cells were then studied for expression of the adhesion molecules CD31/PECAM, CD34, E-selectin, ICAM-1, L-selectin, LFA-3, P-selectin, and VCAM-1 plus the binding of wheat germ agglutinin lectin. The patterns of adhesion molecule expression and lectin binding by these cells are characteristic of hepatic sinusoidal endothelia. In this paper, we have described a method for isolation and culture of human cells with the morphologic and phenotypic characteristics of hepatic sinusoidal endothelia.

Page Thumbnails

  • Thumbnail: Page 
370
    370
  • Thumbnail: Page 
371
    371
  • Thumbnail: Page 
372
    372
  • Thumbnail: Page 
373
    373
  • Thumbnail: Page 
374
    374
  • Thumbnail: Page 
375
    375
  • Thumbnail: Page 
376
    376
  • Thumbnail: Page 
377
    377