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³¹P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi

Nanna Rasmussen, David C. Lloyd, R. George Ratcliffe, Poul Erik Hansen and Iver Jakobsen
Plant and Soil
Vol. 226, No. 2, ICOM2/COST WORKSHOP DEVELOPMENT AND FUNCTION OF THE HYPHAE OF ARBUSCULAR MYCORRHIZAL FUNGI (AMF) (2000), pp. 245-253
Published by: Springer
Stable URL: http://www.jstor.org/stable/42950896
Page Count: 9
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³¹P NMR for the study of P metabolism and translocation in arbuscular mycorrhizal fungi
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Abstract

³¹P nuclear magnetic resonance (NMR) spectroscopy was used to study phosphate (P) metabolism in mycorrhizal and nonmycorrhizal roots of cucumber (Cucumis sativus L) and in external mycelium of the arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck & Smith. The in vivo NMR method allows biological systems to be studied non-invasively and non-destructively. ³¹P NMR experiments provide information about cytoplasmic and vacuolar pH, based on the pH-dependent chemical shifts of the signals arising from the inorganic P (Pi) located in the two compartments. Similarly, the resonances arising from α, β and γ phosphates of nucleoside triphosphates (NTP) and nucleoside diphosphates (NDP) supply knowledge about the metabolic activity and the energetic status of the tissue. In addition, the kinetic behaviour of P uptake and storage can be determined with this method. The ³¹P NMR spectra of excised AM fungi and mycorrhizal roots contained signals from polyphosphate (PolyP), which were absent in the spectra of nonmycorrhizal roots. This demonstrated that the Pi taken up by the fungus was transformed into PolyP with a short chain length. The spectra of excised AM fungi revealed only a small signal from the cytoplasmic Pi, suggesting a low cytoplasmic volume in this AM fungus.

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